The Particle Technology Forum 

Standard Reference Materials
for Particle Size Analysis

Latest Changes: 05Jul27 - go to grey borders / 06Sep13 - revise wording of introduction / 08Jan10 - NIST Size Standards /

ISO Standards for Size Analysis | Particles with Certified Distributions
Criteria for Inclusion on this page | Back to HOME PAGE

This Web page is hosted by the Particle Technology Forum (a division of the American Institute of Chemical Engineers).

Its purpose is to provide a long-term, regularly-updated catalog of links to sources of Certified Reference Materials (CRMs) of particles that have certified size distributions.

Suggestion: To search for a CRM made of a specific material use your browser search function. For example, using Internet Explorer, click on "Edit", then "Find on this Page", and then type the name of the material you are looking for.

International Standards for Particle Size Analysis

The International Standards Organization (ISO)  develops and publishes standard procedures that are accepted by many governmental and commercial organizations as the basis for construction, production, and quality assurance.

The ISO subcommittee on Size Analysis by Non-sieve Methods (ISO/TC 24/SC 4) has formulated standards for sizing by
      gravity sedimentation = ISO 13317
      centrifugal sedimentation = ISO 13318
      electrical sensing zone = ISO 13319
      laser diffraction = ISO 13320
      photon correlation spectroscopy = ISO 13321
      image analysis = ISO 13322
      single particle light interaction = ISO 13323
      x-ray scattering = ISO 13762 You may also search the ISO site using "particle size analysis".

ISO standards often specify that size-measuring instruments should be calibrated by or checked against Certified Reference Materials (CRMs) of particles having certified size distributions. The International Standards Organization itself does not provide CRMs, so people who wish to calibrate their instruments must purchase themfrom commercial or government sources.

The names and contact information for these sources change as new CRMs are developed. Consequently, a CRM source list provided in a printed ISO standard may not be helpful for locating a suitable source.

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Sources of Powders with Certified Size Distributions 

Disclaimer: Inclusion of vendors is for informational purposes only and does not constitute or imply any endorsement of their products over others. Similarly, omission of vendors does not constitute or imply any criticism of their products compared to others.

APPIE JAPAN

JAPANESE INDESTRIAL STANDARD JIS Z 8901 defines 28 kinds of powders for industrial testing, such as abrasion or like tests of machinery, performance test of dust collectors, air filters and fuel or lubricant filters for engine. APPIE supplies 26 kinds of JIS test powders. These are well-characterized powders (in the 1.6 to 200 µm range) made of alumina, barium titanate, calcium carbonate, carbon black, fly ash, loam, quartz, soda-lime glass, and talc.

Bangs Laboratories [Note: In 2003 July Bangs Laboratories became a wholly-owned subsidiary of Polysciences, Inc. ]

Spheres from nanometers to millimeters in size
    Narrow Size Distribution
    Plain (Hydrophobic) Polystyrene Microspheres  
    Other Polymer Microspheres  
    Plain (Hydrophilic) Silica Microspheres
    Carboxylate-Modified Silica Microspheres  
    NIST-Traceable Microsphere Kits  
    NIST-Traceable Microsphere in Bulk  
   
Polydisperse Polymer Beads (Large)
Porous Beads  

Microspheres with Various Surface Chemistries  
    Carboxylate-Modified Microspheres  
    Amino-Modified Microspheres  
    Preactivated Microspheres  
    Other Functionalized Microspheres  

Colored Microspheres
    Dyed Polystyrene and Other Polymer Microspheres  
    Dyed Carboxylate-Modified Microspheres  
    Dyed Amino-Modified Microspheres  
    Dyed Other-Modified Microspheres  

Fluorescent Microspheres
    Fluorescent Polystyrene and Other Polymer Microspheres  
    Fluorescent Carboxylate-Modified Microspheres  
    Fluorescent Amino-Modified Microspheres  
    Fluorescent Other-Modified Microspheres  

estapor® SuperParaMagnetic Microspheres (US and Canada)  
    Classical Magnetic Microspheres  
    Encapsulated Magnetic Microspheres  
    Special Magnetic Microspheres  

ProActive® Protein-activated or -Coated Microspheres
    ProActive Microspheres  

Duke Scientific Co.

narrow size distribution
      polystyrene latices: series from 0.020 to 0.9 µm
      silica spheres: a series from 0.5-1.5 µm
      polystyrene latices: series from 1 to 1,000 µm

broad size distribution
      soda glass spheres: series from 2-2,000 µm

Microspheres-Nanospheres Co.

narrow size distribution
      alumina: 80 NIST-traceable dispersions in sizes from 100 nm to 100 µm
      polystyrene and cross-linked Melamine: NIST-traceable dispersions in sizes from 0.1 to 15 µm
      silica: 80 NIST-traceable dispersions in sizes from 50 nm to 100 µm

National Institute of Science and Technology (U.S.)

narrow size distribution
      polystyrene latices: about 0.1, 0.3, 3, 10, and 30 µm

broad size distribution
      silicon nitride (not spheres): 0.2-10 µm
      soda glass spheres: 10-60, 40-170, 100-400, 220-750, 750-2450 µm
      zirconium oxide (not spheres): 0.3-2 µm, 10-150 µm

Polysciences, Inc. paramagnetic microspheres with a polystyrene surface and fluorescent particles.

NIST Traceable Particle Size Standards are dispersions of polystyrene microspheres that have been measured on in-house instruments calibrated with NIST (National Institute of Standards and Technology) Standard Reference Materials. They are produced with a narrow size distribution and offered in many sizes. Each product is supplied with a Certificate of Analysis. The size, standard deviation, coefficient of variation, and lot number are printed on the label for easy reference. They are packaged in convenient, easy to use 15ml dropper bottles at 1% solids (w/v) in an aqueous suspension.
Nanobead Standards: 40, 50, 60, 70, 80, 90, 100, 125, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900 and 950nm
Microbead Standards: 1.00, 1.25, 1.75, 2.00, 2.50, 3.00, 3.50,4.00, 5.00, 6.00, 7.00, 8.00, and 9.00 µm
Megabead Standards: 10.0, 12.0, 15.0, 20.0, 25.0, 30.0, 40.0, 50.0, 60.0, 80.0, 100.0, 125.0, 150.0, and 175.0µm

Polybead® Polystyrene Microspheres - in aqueous suspension and dry form

Polystyrene Microspheres, Functionalized
    Amino Microspheres
    Carboxylate Microspheres
    Carboxy-Sulfate Microspheres
    Hydroxylate Microspheres
    Sulfate Microspheres
    Chloromethyl Microspheres

Fluorescent Microspheres
    Fluoresbrite® Fluorescent Polystyrene Latex Microspheres
    Fluoresbrite® Fluorescent Carboxylate Microspheres
    Fluoresbrite® Multifluorescent Microspheres
    Polymerizable Fluorescent Microspheres

FlowCheck® Microspheres
    Dyed Polystyrene Microspheres
    Dyed Carboxylate Microspheres
    Protein Bound Microspheres
    Biotin Coated Microspheres
    Streptavidin Coated Microspheres

Kits for Flow Cytometry
    Polystyrene, Amino, Carboxylate Sampler
    Fluorescent Size Range
    Fluorescent Flow Cytometer Calibration Grade
    Dyed Sampler
    Protein Coupling for large and small Microspheres

Superparamagnetic Particles

Additional Polymer Microspheres
    Biodegradable
    Acrylate
    Crosslinked Melamine
    Hollow
    Polymethyl methacrylate
    Polyballs (polypropylene, polyethylene, Teflon, polystyrene)
    Inorganic Particles (glass, silica)
    Natural Biological Particles (pollen, lycopodium)

Reade Advanced Materials has an extensive line of (primarily inorganic) powders: metals, metal oxides, sulfides, sulfates, carbonates, silicates, etc.

Whitehouse Scientific

Monosphere Standards
Intermediate Standards
Broad Range Standards
Ultra-wide Range Standards
Single Sieve Standards
Wide Ranage Sieving Standards
General Purpose Sieve Fractions

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Criteria for Inclusion 

A vendor who wishes to have products listed here should meet the following criteria:
  • Use a powder or slurry whose physical parameters (density, refractive index, etc.) are known and uniform for all particles in the sample.

  • Measure and certify the particle size distribution using a clearly defined technique -- preferably a method that allows tracing the certification to primary standards

  • Sell the standard reference material to anyone who wishes to buy it. Do not limit sales to members of a specific organization or nation.

  • Provide the buyer with a certificate listing the physical parameters (density, refractive index, etc.) and the particle size distribution so that data reduction programs in the size analysis instruments can be provided with accurate values for these parameters.
If you wish to have your products included on this Web page send a note describing your standard reference materials along with the URL of your Web site (or your mailing address) to the PTF Web site manager (see below).

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You may Email the PTF Web Site Manager at
URL: http://www.erpt.org/ptf/partstds.htm